Inspired by Kim Cofino's article in Technology & Learning, please add to this list!

• knowing to hold your mouse over an icon or a link to see what it does.
• understanding that the menus for any program are at the top of the screen, that they are usually very similar, and generally what you find within them (for example: “view” usually means how you see things on the screen and that menu is found in almost every program).
• recognizing that when something is lit up (or underlined) on a website, you can click on it.
• knowing that the cursor changes when held over different parts of the screen and what that means (the little arrow turning into a hand over a weblink for example, or being able to stretch out a picture when it turns into the double-sided arrow).
• using tab to move from cell to cell or box to box on forms or websites.
• being able to recognize drop-down menus – and that they hold additional features.
• understanding that right clicking on things brings up more options.
• how to use keyboard shortcuts such as cut, paste, copy, duplicate a document on the desktop, switch applications / windows  / tabs, etc. (and how to perform these tasks in the first place let alone using the shortcuts!!)
• understanding file structures/hierarchies (nested folders) -- where to save a file, how to save it, what name to give it, and then where to find it again
• ability to rename and move (organize) files and folders
• how to select sections of text (or file icons) by using click and shift-click, and how to select non-contiguous icons with control-click.
• on a PC, understanding how to set your system preference to single-click instead of double-click (and the implication that with a single-click preference you "hover" to select items).
• understanding the system-wide preference for viewing folder contents with different views (icons, details, etc.) and that you can sort by them.
• understanding that in most programs that display a list of items (file system, email, etc.) you can sort those items by clicking on the column headers.
• knowing the use of the "Backspace" or "Delete" key in a web browser takes you back to the previous webpage (and space bar is like page down)
• recognize the right type of font for the audience (ie. sans-serif fonts for web and screens, serif for paper based publications).
• able to select an application other than the default to open a file.
• able to convert files (images, documents, video) between different formats.
• able to apply the golden rule of troubleshooting "is it plugged in?"
• understanding when to "open" a file vs. "import" a file; students need to understand which file type an application can open and which file types must be imported into that application; e.g., MovieMaker can open a MovieMaker project, but pictures, sound and audio to build that project must be imported.
• able to refresh a webpage and force a refresh from a non cached version.